B7-33 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Work the dose out from body weight
Syringe size:
Possible vial strengths:
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Example B7-33 Titration Schedule
| Item | Details |
|---|---|
| Standard dose | 200–400 mcg once daily |
| Courses | 10–20 days, 2–3× per year |
| Originator dose | 10–1000× lower (Russian tradition) |
No human trials; single-lab rodent pharmacology (relaxin B-chain analog). The '1–5 mg per dose' figure is a recurring placeholder that never matched a validated protocol for any bioregulator; sources disagree up to 1000× on real doses.
Possible vial strengths:
What Is It?
B7-33
Single-chain relaxin analogue.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix B7-33
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
B7-33 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
B7-33 (CAS 1818415-56-3) is a 24-amino-acid single-chain peptide derived from the B-chain of the hormone H2 relaxin, designed as a simplified relaxin-2 analog. It acts as a "biased" agonist at the RXFP1 receptor, switching on relaxin's anti-fibrotic signaling while avoiding the pathway tied to its vascular and tumor-promoting effects. All evidence to date is preclinical — cells and rodents only.
In animal and cell studies, B7-33's main reported effect is reduced fibrosis (scarring) in heart, lung, and kidney tissue, achieved by triggering MMP-2 and breaking down excess collagen. Because it favors the pERK1/2 pathway over the cAMP route of full relaxin, researchers use it as a tool to study anti-scarring signaling without relaxin's proliferative effects. These are preclinical findings, not demonstrated outcomes in humans.
There is no established human dose for B7-33 — it has never been through human trials — so any figure is a research choice, not a recommendation. The reconstitution math itself is standard: a 5 mg vial + 2 mL bacteriostatic water = 2.5 mg/mL, so a 250 mcg amount = 0.1 mL (10 units on a U-100 insulin syringe). Larger water volumes give lower concentration and smaller draws for the same amount.
Native H2 relaxin (the recombinant drug serelaxin) is a complex two-chain, disulfide-linked molecule that is costly to make and whose cAMP signaling is linked to tumor-promoting effects — and serelaxin itself failed its phase 3 heart-failure trial. B7-33 was engineered as a single-chain shortcut that keeps the anti-fibrotic action while shedding that cAMP arm, making it cheaper to synthesize and cleaner mechanistically. So they are not interchangeable: serelaxin is full-length relaxin, B7-33 is a truncated biased agonist.
B7-33 is not approved anywhere — no FDA approval, no human safety data, and no clinical trials — so despite the strong anti-fibrotic reputation online, its profile in people is simply unknown. It is sold strictly as a research-grade compound, and "research-grade" is never the same as a tested medicine. Storage: keep the lyophilized powder refrigerated and protected from light, and use any reconstituted solution promptly rather than storing it long-term.
Practical takeaway: If your goal involves weight management, metabolic health, recovery, physical performance, cognitive function, appearance, or general well-being, discuss it with a licensed healthcare professional. They can help you evaluate approved, evidence-based options rather than relying on an unapproved or investigational compound. This calculator is intended only for transparent research-reference calculations and does not determine an appropriate amount, recommend use, or replace professional medical guidance.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.