TB-500 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
⚠ Use acetic acid — not bacteriostatic water
TB-500 is insoluble or unstable in standard BAC water. Manufacturer protocols require reconstitution in dilute acetic acid (0.1%) — or, for IGF-1 variants, 50 mM acetic acid. Volumes shown below refer to acetic acid, not water.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example TB-500 Titration Schedule
| Protocol Item | Guidance |
|---|---|
| Standard Dose Range | 300–1000 mcg per dose |
| Higher-Dose Note | 2–3 mg has been used in severe injuries (reported positive effects) |
| Frequency | Daily |
| Typical Cycle Length | 30 days (can be extended up to 90 days) |
| Minimum Cycle Break | 30-day minimum break between cycles |
| Adjustment Note | Cycle length and intensity depend on response and goal |
Possible vial strengths:
What Is It?
TB-500
Synthetic thymosin beta-4 fragment.
Acetic Acid (0.1%)
A dilute solution of acetic acid (typically 0.1% for most peptides, or 50 mM for IGF-1 variants) used to reconstitute peptides that are insoluble or unstable in standard bacteriostatic water. TB-500 requires an acidic carrier to dissolve fully and remain stable — plain BAC water will not work and may damage the peptide.
How To Mix TB-500
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw Acetic AcidDraw the selected amount of 0.1% acetic acid.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
TB-500 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
TB-500 is a synthetic fragment of thymosin beta-4 (Tβ4), a natural repair protein found in nearly every cell. The fragment keeps Tβ4's active actin-binding region and is studied as a "healing" peptide — not a steroid, and not a hormone.
In research and anecdotal use it's referenced in milligrams — commonly around 2–5 mg per week by subcutaneous injection, often split into smaller doses, with a higher "loading" period followed by less frequent maintenance. These are reference figures, not an approved regimen, and dosing is a clinical decision.
Add bacteriostatic water and swirl gently — don't shake. A 5 mg vial + 2.5 mL gives 2 mg/mL (2,000 mcg/mL), so a 2 mg dose = 1 mL = 100 units on a U-100 syringe. Use the calculator above for your exact vial size.
They heal through different mechanisms: TB-500 works by regulating actin and cell migration, acting systemically throughout the body, while BPC-157 leans on blood-vessel growth and works more locally. TB-500 shows stronger animal signals in muscle, skin, and cardiac tissue; unlike BPC-157 it has no oral form, so it's injected. How anyone pairs them is a clinical decision, not something a calculator should merge.
Most of TB-500's reputation rests on animal studies — and importantly, the stronger human data is for full-length thymosin beta-4, not the TB-500 fragment that's actually sold. There's no human evidence it causes cancer, though its role in cell migration and vessel growth is a theoretical caution. It's not FDA-approved and is banned by WADA. Store the dry vial refrigerated and dark; once mixed, keep cold and use within a few weeks.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.