Follistatin-344 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example Follistatin-344 Titration Schedule
| Protocol Item | Guidance |
|---|---|
| Dose | 100–300 mcg per dose |
| Cycling | 8 weeks on, 4–8 weeks off |
| Administration Note | Some theorize that injecting into the targeted muscle is best. |
Possible vial strengths:
What Is It?
Follistatin-344
Alternative follistatin isoform.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix Follistatin-344
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
Follistatin-344 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Follistatin-344 (FS-344) is the longer precursor form of follistatin, a protein that blocks myostatin — the body's brake on muscle growth. It's the most commonly sold research form, and the body processes it into the circulating FS-315.
In research protocols it's referenced around 100 mcg per day by subcutaneous injection, usually in short blocks of a few weeks. These are reference figures, not an approved regimen, and dosing is a clinical decision.
Add bacteriostatic water and swirl gently — don't shake. A 1 mg vial + 1 mL gives 1 mg/mL (1,000 mcg/mL), so 100 mcg = 0.1 mL = 10 units on a U-100 syringe.
No. Gene therapy delivers a gene (AAV-FS-344) so your own muscle keeps making follistatin for months; injecting FS-344 peptide just adds a dose that clears within hours. The dramatic before-and-after results people search for come from the gene-therapy route, not from injecting the peptide.
The "double-muscled" reputation comes from genetic models, not from injecting the peptide, which has little human evidence. It's not FDA-approved and is banned by WADA as a myostatin inhibitor; blocking activin also raises theoretical reproductive-hormone effects. Store the dry vial refrigerated and dark; once mixed, keep cold and use within a few weeks.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.