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Follistatin-344 and BAC Water Mix

Follistatin-344 Reconstitution Calculator

Reviewed by · Pharmacist and Scientific Reviewer

Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.

What amount do you need?

Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.

Work the dose out from body weight

Syringe size:
Possible vial strengths:
Best Match Good Match Usable Harder to Measure

Example Follistatin-344 Titration Schedule

ItemDetails
Community dose100–300 mcg 2–3× per week
Cycle8 weeks / 4–8 off

FS-344 is the precursor construct used in gene-therapy trials (AAV1.CMV.FS344) — the positive human results (BMD: 6MWT +29–125 m; sIBM: +56 m/yr) belong to intramuscular gene expression of FS-344, not to injected peptide. No injectable FS-344 peptide has been tested in humans.

Possible vial strengths:

What Is It?

Follistatin-344

Alternative follistatin isoform.

Bacteriostatic Water

Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.

How To Mix Follistatin-344

1
Clean

Use alcohol swabs to clean the tops of both vials.

2
Draw BAC Water

Draw the selected amount of bacteriostatic water.

3
Inject Slowly

Add the liquid slowly down the side of the vial.

4
Swirl Gently

Do not shake. Swirl gently until dissolved.

5
Store Properly

Store as directed and protect from heat and light.

Best Practices & Common Mistakes

Best Practices

  • Use sterile technique.
  • Protect from light and heat.
  • Store refrigerated when appropriate.
  • Use clean syringe-unit math before measuring.

Common Mistakes

  • Confusing milligrams with milliliters.
  • Choosing an option with awkward decimal units.
  • Using too little liquid for very small measurements.
  • Shaking the vial aggressively.

Follistatin-344 Storage & Handling

Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.

Frequently Asked Questions

Follistatin-344 (FS-344) is the longer precursor form of follistatin, a protein that blocks myostatin — the body's brake on muscle growth. It's the most commonly sold research form, and the body processes it into the circulating FS-315.
In research protocols it's referenced around 100 mcg per day by subcutaneous injection, usually in short blocks of a few weeks. These are reference figures, not an approved regimen, and dosing is a clinical decision.
Add bacteriostatic water and swirl gently — don't shake. A 1 mg vial + 1 mL gives 1 mg/mL (1,000 mcg/mL), so 100 mcg = 0.1 mL = 10 units on a U-100 syringe.
No. Gene therapy delivers a gene (AAV-FS-344) so your own muscle keeps making follistatin for months; injecting FS-344 peptide just adds a dose that clears within hours. The dramatic before-and-after results people search for come from the gene-therapy route, not from injecting the peptide.
The "double-muscled" reputation comes from genetic models, not from injecting the peptide, which has little human evidence. It's not FDA-approved and is banned by WADA as a myostatin inhibitor; blocking activin also raises theoretical reproductive-hormone effects. Store the dry vial refrigerated and dark; once mixed, keep cold and use within a few weeks.
Practical takeaway: If your goal involves weight management, metabolic health, recovery, physical performance, cognitive function, appearance, or general well-being, discuss it with a licensed healthcare professional. They can help you evaluate approved, evidence-based options rather than relying on an unapproved or investigational compound. This calculator is intended only for transparent research-reference calculations and does not determine an appropriate amount, recommend use, or replace professional medical guidance.

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References

  1. Mendell JR, et al. AAV follistatin in BMD/sIBM. Mol Ther. 2015.

About the author

· Pharmacist and Scientific Reviewer

Maria Hrabina is a pharmacist and scientific reviewer with a strong interest in peptide science, pharmacology, and emerging therapeutic compounds.

Her work focuses on understanding how peptide-based compounds interact with biological signaling pathways and how they are being investigated across areas such as metabolic health, dermatology, recovery, regenerative research, and pharmaceutical development.

Maria follows research on a broad range of peptides, from compounds used in approved medicines to experimental molecules still being evaluated in laboratory, preclinical, and clinical studies.

Her approach combines scientific curiosity with critical evaluation of evidence. She believes promising mechanisms and early research deserve attention, but should not be presented as established clinical benefits until they are supported by adequate human data.

Her areas of focus include scientific literature review, mechanisms of action, pharmacology, pharmacokinetics, safety, pharmaceutical formulation, and the overall quality of evidence behind peptide-based compounds.

She is particularly interested in the process of translating an experimental molecule into a reliable pharmaceutical product — including compound characterization, purity, stability, formulation, dose selection, safety assessment, and controlled clinical trials.

Maria believes peptide science has significant long-term potential, but that its progress depends on rigorous research, high manufacturing standards, transparent safety data, and responsible scientific communication.

Through her writing, she aims to help readers understand both sides of peptide research: what makes these compounds scientifically promising and what questions remain before their potential can be considered clinically established.

Areas of interest: Peptide Science · Therapeutic Peptides · Peptide Pharmacology · Metabolic Peptides · Cosmetic Peptides · Drug Development · Pharmacokinetics · Drug Safety · Pharmaceutical Formulation · Emerging Therapies · Evidence-Based Medicine

Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.
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