Epithalon (Epitalon) Reconstitution Calculator

Yes — Epitalon, Epithalon, and Epithalone are three spellings of the same synthetic tetrapeptide, Ala-Glu-Asp-Gly (AEDG), and the variation just comes from transliterating the original Russian name. Don't confuse it with Epithalamin, the bovine pineal-gland extract it was modeled on — much of the human longevity data people cite actually used that extract, not the synthetic peptide. It's also a different molecule from Pinealon (Glu-Asp-Arg), a separate Khavinson peptide aimed at neurons rather than the pineal-telomerase axis.

Only the lyophilized (freeze-dried) injectable powder gets reconstituted — add bacteriostatic water down the vial wall, swirl gently, and don't shake. As a worked example, a 50 mg vial plus 4 mL of bacteriostatic water gives 12.5 mg/mL; a 10 mg research dose is then 0.8 mL, which reads as 80 units on a U-100 insulin syringe. Add more water if you'd prefer a lower concentration and an easier-to-measure dose.

The most commonly cited preclinical protocol is 5–10 mg per day by subcutaneous injection for 10–20 consecutive days, repeated two to three times a year rather than run continuously. That short pulsed schedule reflects how the peptide was studied in the Khavinson work, not a validated human regimen. Since there's no approved dosing, any figure you find online is a research reference point, not a recommendation.

In cell studies, Epithalon raised expression of hTERT — the catalytic subunit of telomerase — and lengthened telomeres, with a 2025 independent replication reproducing the telomere effect in normal human cell lines. It's also studied for restoring pineal melatonin output and normalizing circadian rhythm, since it derives from a pineal-gland peptide. One honest caveat: because telomerase activation could in theory support proliferation of cells that already have dysregulated growth, the theoretical cancer question is the main safety discussion point, and it hasn't been settled in humans.

No — the calculator only applies to the injectable lyophilized powder. Capsules and sublingual tablets are taken as-is, and nasal sprays come either pre-formulated or mixed-to-order by the vendor with saline, so there's nothing for you to reconstitute. N-Acetyl Epithalon Amidate is a stability-modified version (N-acetylated and C-terminal amidated) sold as both a ready nasal spray and an injectable powder — only that injectable powder goes through the same reconstitution steps.