PE-22-28 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Work the dose out from body weight
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example PE-22-28 Titration Schedule
| Item | Details |
|---|---|
| Community dose | 2–10 mg per dose (clinician-reported) |
| Rodent dose | 3–4 µg/kg IP |
| Pattern | Continuous (antidepressant class) |
A chronic-disease treatment is not cycled in 30-day blocks — no protocol or pharmacological rationale supports it; rodent efficacy used continuous sub-chronic dosing. The listed 200–600 mcg matches only the raw rodent transposition; clinician-reported community use runs 2–10 mg. Zero human trials; one lab's preclinical work.
Possible vial strengths:
What Is It?
PE-22-28
Spadin analogue studied for mood models.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix PE-22-28
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
PE-22-28 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
PE-22-28 is a synthetic seven-amino-acid peptide taken from a piece of spadin — a natural fragment of the sortilin protein — and its job is to block the TREK-1 potassium channel in the brain. That mechanism is the whole point: mice lacking TREK-1 are resistant to depression, so inhibiting the channel produces fast antidepressant-like effects in rodent tests, and PE-22-28 does it far more potently than spadin itself while also boosting hippocampal neurogenesis. It's a genuinely novel approach compared with SSRIs, ketamine, or psychedelics — but so far the evidence is entirely preclinical.
It comes as a lyophilized powder, so add bacteriostatic water down the vial wall, swirl gently, and don't shake. As an illustrative example, an 8 mg vial with 6 mL of bacteriostatic water gives 1.33 mg/mL, so a 400 mcg dose would be 0.3 mL — 30 units on a U-100 insulin syringe. Since there are no human dosing studies, treat any figure like this as extrapolated from animal work rather than an established amount.
In the research, PE-22-28 and its parent spadin were given by injection, and that's the form this calculator covers — the reconstituted lyophilized powder. It's also sold as a pre-made nasal spray, which is dosed per spray and isn't something you reconstitute yourself; nasal delivery is often chosen for peptides to ease brain access and skip injections. Which route makes sense is a separate question from the mixing math, and neither has validated human dosing behind it.
All three get lumped together as "mood and brain" peptides, but they act through completely different targets. PE-22-28 blocks the TREK-1 potassium channel as an antidepressant strategy, Selank is a tuftsin-derived anxiolytic that modulates GABA (calming rather than mood-lifting), and Dihexa works on the HGF/c-Met pathway aimed at synapse formation and cognition. They aren't interchangeable, so if you're comparing them it's worth reading each on its own terms rather than treating them as versions of one thing.
Because PE-22-28 has only been studied in animals, its side-effect profile in humans is essentially unknown, and no completed clinical trials establish that it's safe or effective. It's important to be clear that this is an early-stage research compound, not a treatment for depression — depression is a serious medical condition that warrants care from a qualified professional, and PE-22-28 is not a substitute for that. It isn't FDA-approved, and blocking a channel involved in mood, pain, and other functions simply hasn't been characterized for safety in people.
Practical takeaway: If your goal involves weight management, metabolic health, recovery, physical performance, cognitive function, appearance, or general well-being, discuss it with a licensed healthcare professional. They can help you evaluate approved, evidence-based options rather than relying on an unapproved or investigational compound. This calculator is intended only for transparent research-reference calculations and does not determine an appropriate amount, recommend use, or replace professional medical guidance.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.